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The mRNA abundance of CrCIS gene and corresponding biomass and lipid content of the cells in HSM and HSM-N media. The mRNA levels were analyzed through RT-PCR of C. reinhardtii CC425 samples grown in the indicated media for 1, 2, 3, or 4 d. +N: cells cultivated in N sufficient HSM medium; -N: cells cultivated in N free HSM medium.
