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A typical trace of respirometric measurements of mitochondria isolated from brain of normal piglets. Substrates were 6 mM pyruvate + 6 mM malate. Further additions: MX - 0.25 mg of mitochondria; ADP - 2 mM ADP; Amit - 2 mM amytal; Succ – 10 mM succinate; ATR – 0.1 mM atractyloside; Cyt c - 32 μM cytochrome c. The blue trace indicates oxygen concentration, the red trace – oxygen flux.
