Table 3.
The species for which alternative primers have been used for DNA amplification.
| Taxon (no specimens) | Probable reason for failure | Used primer(s) | Additional sequences with Folmer et al. (1994) |
|---|---|---|---|
| Acanthiophilus walkeri (1) | DNA degraded, specimen stored in ethanol 70% for 7 years | All | 0 |
| Bactrocera oleae (1) | DNA degraded, specimen stored in ethanol 70% | All | 1 |
| Plaumannimyia sp. (1) | ? | TEPCOI | 0 |
| Rhagoletis cerasi (1) | ? | TEPCOI | 4 |
| Rhagoletis cingulata (3) | Taxon-specific mutation at primer site? | TEPCOI | 0 |
| Rhagoletis samojlovitshae (1) | DNA degraded, specimen stored in ethanol 70% for 10 years | All | 0 |
| Sphenella marginata (7) | Taxon-specific mutation at primer site? | TEPCOI, TEP_F2, TEP_R2 & Folmer et al. (1994) | 0 |
| Tephritis nebulosa (1) | DNA degraded, specimen stored in ethanol 70% for 10 years | All | 0 |
| Terellia colon (1) | ? | TEPCOI | 11 |
| Terellia luteola (1) | DNA degraded, specimen stored in ethanol 70% for 10 years | TEPCOI, TEP_F2, TEP_R2 & Folmer et al. (1994) | 1 |
| Trupanea cf. metoeca (1) | DNA degraded, specimen stored in ethanol 70% for 2 years | TEPCOI | 0 |
| Trypeta artemisiae (2) | ? | TEPCOI | 1 |
| Ulidia nigripennis (1) | ? | TEPCOI | 0 |
| Urophora ivannikovi (1) | DNA degraded, specimen stored in ethanol 70% for 10 years | All | 0 |