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Confirming isolation of exosomes by ultracentrifugation. (A) Scanning electron micrograph of U87MG cell surface with vesicular protrusions (arrows; scale bar, 250 nm) and (B) transmission electron micrograph of purified U87MG exosomes (scale bar, 100 nm). (C) U87MG-GFP cells with PKH26-labeled exosomes in the cytoplasm and on the cell surface (white arrows). (D) Immunoblot analysis shows presence of exosome markers in U87MG exosomes and parent cell line.
