Figure 3.

Radiation-derived exosomes are more readily taken up by recipient cells. (A) Confocal microscopic visualization of exosomes within cells. Exosomes are stained red with PKH26, and nuclei are stained blue with DAPI. (B) Flow cytometric quantitation of exosome uptake by cells after 1 to 24 hours in coculture. (C and D) Radiation-derived exosome uptake (4-Gy exosomes) in recipient cells at 24 hours compared with exosomes from nonirradiated cells (0-Gy exosomes) incubated for the same time; (D) is the averaged value + SEM of three independent experiments, relative to the 0-Gy condition. *P = .03.