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. 2013 Dec 1;6(6):676–684. doi: 10.1593/tlo.13475

Figure 1.

Figure 1

EV20 inhibits ErbB-3/Akt activation in both a ligand-dependent and a ligand-independent manner. To study the effect of EV20 on ligand-dependent activation of ErbB-3/Akt, DU-145, IR-8, and OVCAR-8 cells were serum starved for 24 hours and then treated with increasing doses of EV20 for 2 hours, before stimulation with 10 ng/ml NRG-1β (5 minutes). For ligand-independent assay, the serum-starved (24-hour) Skbr-3 cells were treated with increasing doses of the antibody for 2 hours and then harvested. Lysates obtained from cells were immunoblotted as indicated. Immunoreactivity was quantified and plotted after normalization to total Akt. Data are presented as percentage of inhibition of ErbB-3/Akt phosphorylation in EV20-treated samples over control. The presented data are representative of two independent experiments.