Fig. 2.

IL-25 induces pulmonary collagen deposition in WT but not Rora^sg/sg mice. WT and Rora^sg/sg were treated with recombinant mouse IL-25 i.n. on days 0, 3, 7, 10, and 13. (A) Representative histology images depicting collagen deposition within the lung using Masson’s Trichrome stain (scale, 100 μM). (B) Total collagen in lung digests expressed per mg of lung protein. (C) Levels of IL-13, IL-17A, and TGFβ in lung digests expressed per mg of lung protein. Data are representative of mean ± SEM (n = 3–5) from two individual experimental replicates (ns, not significant; *P < 0.05, **P < 0.01).