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Zn(II)-dependent fluorescence signal enhancement of DA-ZP1-TPP. Normalized fluorescence spectra of a 1 µM solution of DA-ZP1-TPP in buffer (50 mM PIPES, 1 mM EDTA, 2 mM CaCl2, and 100 mM KCl; pH 7) on increasing concentrations of free zinc ions. (Inset) Zinc-binding isotherm normalized to the integrated fluorescence signal intensity under zinc-saturating conditions.
