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Kinetic traces for the deacetylation of DA-ZP1-TPP. The half-life for deacetylation of a 2.75 µM solution of DA-ZP1-TPP at 37 °C in buffer (50 mM PIPES, 100 mM KCl; pH 7), was measured in the presence of 125 µM ZnCl2 (black circles), 250 µM EDTA (triangle), or 1.25 U of porcine liver esterase (white circles). The change in absorbance at 520 nm (EDTA and esterase) or 510 nm (ZnCl2) was used to monitor the reaction.
