Table 1.
Lipophilicity, charge of predominate species in solution at physiological pH, and observed cellular localization of various dyes in live HeLa cells
| Compound | log P value* | Charge† | Localization |
| MitoTracker Red | 1.15 ± 0.04 | +1 | Mitochondria |
| DA-ZP1-TPP | 0.74 ± 0.18 | +1 | Mitochondria |
| C343-TPP | 0.72 ± 0.05 | +1 | Mitochondria |
| MitoTracker Green | 0.56 ± 0.02 | +1 | Mitochondria |
| ZP1-TPP | −0.11 ± 0.01 | Zwitterion | Endosome/lysosome |
| FL-TPP | −0.39 ± 0.03 | −1 | Extracellular |
*
Log P values were measured in octanol/water, buffered at pH 7 with 10 mM Tris, using a modified shake-flask procedure (SI Appendix).
†
Charge of the predominate species in solution at pH 7.4.