Fig. 4.

bfRAG1L can recognize RSS after reconstitution. (A) Scheme of the constitution of Ch-bfRAG1L. (B) Co-IP assay to examine the interaction between Ch-bfRAG1L and cRAG2 in HEK-293T cells. IB, immunoblot; nt, N-terminal. (C) The recognition of RSS by Ch-bfRAG1L. The “fold transactivation” of luciferase activity was normalized against ”no RAG control” with the indicated plasmids (the means ± SD is calculated from triplicate experiments). Error bars reflect the SD. Asterisks indicate the P value by using the Student t test (*P < 0.05; **P < 0.01). (D) The nuclease activities of bfRAG1L and Ch-bfRAG1L on a genomic DNA substrate. Ctrl, represent untreated genomic DNA from 293T cell lines. The results are representative of two (B) or four (D) independent experiments.