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Overall fold of APIP/MtnB and its tetrameric assembly. (A) The monomeric APIP/MtnB structure is sketched in ribbon representation. An active site zinc ion is shown in a gray ball. (B) Tetramer assembly of APIP/MtnB in C4 symmetry is observed in an asymmetric unit of the crystal lattice. Zinc ions are represented as balls colored similarly as the corresponding subunits embracing them. (C) SEC-MALS shows that APIP/MtnB exists as a tetramer in solution.
