Fig. 2.

P_f inhibitory response triggered by cytosolic acidification for FaPIP1;1–FaPIP2;1 (at a 3:1 cRNA mass ratio) and for FaPIP2;1 expressed alone. Shown is P_f vs. [H⁺] inhibitory response profile for oocytes injected with FaPIP2;1 cRNA and oocytes injected with FaPIP1;1 and FaPIP2;1 cRNA in a 3:1 mass ratio. The amount of cRNA is represented in parentheses with an arbitrary unit of measure where 1 is equivalent to 1.25 ng of cRNA. The data points are representative values obtained from the same batch of oocytes (mean P_f ± SEM). The data were fitted to an allosteric sigmoidal dose–response curve. (Inset) For each K_I ([H⁺] at which half of the maximum effect occurs), an EC₅₀ value was calculated using . The mean EC₅₀ value (EC₅₀ ± SEM, n = 3–5) was calculated for each treatment.