Fig. 5.

NLRP12 suppresses NF-κB and ERK activation in response to Salmonella LPS but not to flagellin or the SPI-1 or SPI-2 type III secretion system. BMDMs from WT and Nlrp12^−/− mice were infected with S. typhimurium ΔsipB (A), Δspi-2 (B), and ΔfliCΔfljB (C) (MOI of 5) or with Salmonella LPS (D). Cell lysates were analyzed for P-ERK, ERK, P-IκBα, and IκBα by Western blotting. Data are representative of at least three independent experiments.