Fig. 5.

Further characterization of C. intermedia gxs1 Phe³⁸Ile³⁹Met⁴⁰ triple mutant. (A) Glucose uptake at high cell density for S. cerevisiae EX.12 expressing wild type, Phe³⁸Ile³⁹Met⁴⁰, and empty vector. (B) Xylose uptake at high cell density for S. cerevisiae EX.12 expressing wild type, Phe³⁸Ile³⁹Met⁴⁰, and empty vector. (C) Inhibition of the growth rate on xylose with increasing glucose concentration. (D) V_max of both the wild type and the mutant. (E) K_M of both the wild type and triple mutant. (A–E) Error is based on SD of biological replicates.