Fig. 5.

Low IL-6 secretion by AOAH-deficient cLP DCs abates Th17 polarization. (A) Percentage of positive cells and mean fluorescence intensity for indicated molecules on Aoah^+/+ (black) and Aoah^−/− (red) CD103⁺CD11b⁺ALDH⁻ cLP DCs. Isotype control antibodies are shaded. (B) Supernatant IL-6 from Aoah^+/+ (dark bars) and Aoah^−/− (white bars) CD103⁺CD11b⁺ALDH⁻ cLP DCs cultured overnight without or with LPS (400 ng/mL). (C) Supernatant IL-17 from OT-II cell cultures with Aoah^+/+ and Aoah^−/− CD103⁺CD11b⁺ALDH⁻ cLP DCs loaded with OVA with or without LPS (400 ng/mL). Indicated cultures included neutralizing antibodies against IL-6 and IL-6R (αIL-6; 20 μg/mL each) or rmIL-6 (100 or 400 pg/mL). Data are shown as flow cytometry histograms or mean ± SD of triplicate samples and are representative of at least three independent experiments.