Fig. 5.

DC- and MyD88-dependent signals regulate FRC growth during the immune response. The total cellularity, number of FRCs, and proliferation of FRCs were measured in six draining LNs by flow cytomety at day 3 (B and D) or day 5.5 (A, C, and E) after immunization. (A) Mice that received OT T cells (closed circles) or did not receive OT T cells (open circles) were immunized with PBS, Mont, or OVA/Mont. Statistics were calculated on a pool of all data points. (B) CD11c-DTR tg or ntg littermate mice received OT T cells, were injected with a single dose of DT to deplete DCs, and finally were immunized with PBS or OVA/Mont. (C) WT mice were immunized s.c. with WT or MyD88 KO BMDCs activated with either LPS or CpG without loading of OVA antigen. (D) CD11c-DTR tg or ntg littermate mice were treated with a single dose of DT and then immunized s.c. with PBS or WT BMDCs activated with CpG without OVA antigen. (E) WT or MyD88 KO mice were immunized with WT BMDCs activated with CpG without loading of OVA antigen. Data are mean ± SD from at least two experiments, with n ≥3 mice per experiment.