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. 2013 Sep 13;21(2):234–246. doi: 10.1038/cdd.2013.116

Figure 3.

Figure 3

Persistently active PrlR-JAK2-STAT3/5A/5B signaling confers a survival signal. (a) Reduction in PrlR expression by PrlR-specific shRNA hairpins led to a significant increase in the percentage of early apoptotic cells in SSM2 and SSM3, as measured by flow cytometry. Luc, luciferase-specific shRNA. (b) JAK2-specific shRNA hairpins induced apoptosis in SSM2 and SSM3 compared with control Luc shRNA, as measured by flow cytometry. (c) BMS-911543 induced a dose-dependent increase in apoptosis in SSM2 and SSM3. IC50=56 nM for SSM2 and 58 nM for SSM3. (d) Blockade of STAT3 function using a double-stranded oligonucleotide (S3 decoy) induced apoptosis in SSM2 and SSM3. A mutant oligo (S3 mutant) had no significant effect on cell survival. (e) STAT5A/5B knockdown resulted in an increase in early apoptosis over control hairpin-transduced SSM2 or SSM3 cells. *P<0.05, **P<0.01, ***P<0.001. Error bars, S.E.M.