Figure 3.

Deletion of Spry1 disrupts an IL-6/STAT3 autocrine loop. (a) Immunoblot of the indicated (phospho) proteins from pooled samples of the indicated number of thyroid glands. (b) Phosphorylation of STAT3 upon the stimulation of isolated follicles with IL-6 (50 ng/ml, 5 min). (c) Left panel, IL-6 levels secreted by isolated follicles of the indicated genotypes as assessed using ELISA. Right panel, mRNA levels of IL-6 from pooled thyroids of the indicated genotypes. (d) Phospho-STAT3 levels of isolated follicles from wild-type mice incubated for 24 h with the indicated neutralizing antibodies