Figure 4.

Thyroid glands from Spry1 knockout mice exhibit decreased markers of cellular senescence. (a) Cytokine secretion profile of isolated follicles from knockout mice (100% is secretion of wild-type follicles). (b) Left panel, mRNA levels of IGFBP7 from pooled samples of the indicated number of thyroid glands and, right panel, KC levels secreted by isolated follicles of the indicated genotypes. (c) Senescence-associated beta galactosidase activity of wild-type versus Spry1 null thyroids. (d) Silencing of IKKβ reduces the secretion of both IL-6 and KC