Table 1.

The hormone replacements administered to Sham and Ovx mice prior to a cocaine challenge. Shams received vehicle for the same time-frame as hormone-replaced Ovx mice. All injections were given in the morning. All mice received a final injection of vehicle or hormone before being placed into locomotor boxes for habituation. For the 30 min versus 2 day replacement study, mice instead received their final injection after the saline treatment during testing, 30 min prior to the COC injection. E2 = 17β-estradiol; PPT = 4,4′,4″-(4-Propyl-[1H]-pyrazole-1,3,5-triyl)trisphenol; DPN = 2,3-bis(4-Hydroxyphenyl)-propionitrile; N/A = not applicable.

Hormone treatment	Length of replacement	Dose	Injected volume	Activated ER(s)
Vehicle	2 days	N/A	100 μL	N/A
30 min E2	30 min	3 μg once	100 μL	α, β
2 Day/High E2	2 days	3 μg/day	100 μL	α, β
Medium E2	2 days	0.3 μg/day	100 μL	α, β
Low E2	2 days	0.03 μg/day	100 μL	α, β
PPT	2 days	2 mg/kg/day	4 mL/kg	α
DPN	2 days	8 mg/kg/day	4 mL/kg	β