Fig. 1.

Effect of chenodeoxycholic acid (CDCA) on iodide efflux in T84 cells. T84 cells grown in 6-well plates were treated with 0.2% DMSO, cAMP cocktail [100 μM 8-bromo (Br)-cAMP + 10 μM forskolin + 100 μM IBMX; A], CDCA (500 μM), or taurine conjugate TCDC (500 μM; B). Iodide efflux assay was performed as described in materials and methods. Bar graphs show iodide efflux rate (nmol/min), which was calculated over a 2-min interval. Data are expressed as the means ± SE of 3 independent experiments. *P < 0.05, compared with 0.2% DMSO.