Fig. 2.
Effect of CDCA on short-circuit current (Isc) in T84 monolayers in the absence and presence of cytokines. T84 cell monolayers were incubated with a cytokine cocktail containing TNFα (10 ng/ml), interferon-γ (30 ng/ml), and IL-1β (10 ng/ml) for 24 h before being mounted in Ussing chambers. CDCA (500 μM) was first added to the apical chamber and then added to the basolateral chamber. Representative tracing of Isc shows the effect of CDCA in the control (A; n = 3) and cytokines-treated (B; n = 4) T84 monolayers. AM, exposure of apical membrane to CDCA; BLM, exposure of basolateral membrane to CDCA.