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. 2013 Jun 12;305(4):C447–C456. doi: 10.1152/ajpcell.00416.2012

Fig. 5.

Fig. 5.

The role of the cAMP-dependent pathway in the actions of CDCA. A: T84 cells mounted in Ussing chambers were incubated with 50 μM adenylate cyclase inhibitor MDL12330A for 25 min before the basolateral addition of CDCA (500 μM). Bar graph shows the Isc change elicited by CDCA ± MDL12330A. *Statistically significant difference in ΔIsc (P < 0.05) compared with CDCA alone; n = 4. B: T84 cells grown in 96 well-plates were incubated with 0.1% DMSO or 5 μM CDCA or 500 μM CDCA for 20 min, and [cAMP]i was measured using enzyme immunoassay. Bar graph shows the intracellular cAMP concentration ([cAMP]i), expressed in fmole/mg protein. *Statistically significant difference in [cAMP]i (P < 0.05) compared with 0.1% DMSO; n ≥ 4.