Fig. 8.
Role of microtubules in the effect of CDCA on Isc in T84 cells. T84 cells grown in Transwells were pretreated with DMSO (0.1%) or nocodazole (33 μM, bilateral) first on ice for 30 min, and then were mounted in Ussing chambers where they were treated for an additional 30 min at 37°C. CDCA (500 μM) was added to the basolateral solution. Representative tracings of Isc show the effects of CDCA in control (A) and nocodazole (B)-treated T84 cells; n = 3. C: representative blot shows the 0.1% Triton X-100 soluble and insoluble α-tubulin in T84 cells treated with DMSO or nocodazole. Estimated size of the α-tubulin protein is 50 kDa; n = 3.