Fig. 1.

Loss of mixed-lineage kinase 3 (MLK3) reduces diet-induced body weight gain and adiposity. A: wild-type (WT) and MLK3-knockout (KO) mice were maintained on chow (CD) or high-fat diet (HFD) for 16 wk. Body weight was measured monthly. B: after 16 wk on diet, lean and fat mass were determined by MRI. Data are means ± SE; n = 8. *P < 0.05. C–F: CD and HFD mice were monitored for 3 days to measure food consumption (C), physical activity (D), energy expenditure (E), and respiratory quotient (F). Data are means ± SE; n = 8. G: body temperature of WT and MLK3-KO mice is presented. Data are means ± SE; n = 8. H: plasma noradrenaline and adrenaline concentrations were measured on HFD. Data are means ± SE; n = 8. *P < 0.05; ***P < 0.001. NS, not significant.