Fig. 3.

MLK3 deficiency decreases secretion of inflammatory markers. A: plasma concentrations of leptin, monocyte chemoattractant protein-1 (MCP-1), and adiponectin were measured in CD and HFD mice. Data are means ± SE; n = 8. B: IL-6 and MCP-1 in conditioned medium were collected for 24 h from visceral fat explants. Data are means ± SE (n = 4) from 2 different experiments. C: phosphorylation and expression of JNK in epididymal fat pads was examined by immunoblot analysis. Quantification of immunoblot data is shown at right. *P < 0.05; **P < 0.01.