Table 1.
Forward and reverse primers used for quantitative real-time PCR analysis
| Gene | Primer Sequence 5′ to 3′ |
|---|---|
| CTGF | F: AATGTCAGTGCGCAGCCGAAGCA |
| R: AGGGGTCACGCTCCGTACACAG | |
| Fibronectin | F: ACGGTGTCAACTACAAGATCG |
| R: GTCTTCCCATCGTCATAGCAC | |
| TGF-β | F: AGCAACATGTGGAACTCTACC |
| R: GTATTCCGTCTCCTTGGTTCAG | |
| Nox4 | F: TCCAAGCTCATTTCCCACAG |
| R: CGGAGTTCCATTACATCAGAGG | |
| F4/80 | F: CTTTGGCTATGGGCTTCCAGTC |
| R: CAAGGAGGACAGAGTTTATCGTG | |
| TNFα | F: ACGGCATGGATCTCAAAGAC |
| R: CGGACTCCGCAAAGTCTAAG | |
| VCAM-1 | F: ATTTTCTGGGGCAGGAAGTT |
| R: ACGTCAGAACAACCGAATCC | |
| CD3 | F: AGAGCAGCTGGCAAAGGTGGT |
| R: CAGCCATGGTGCCCGAGTCTAGC | |
| CD4 | F: TCACCTGGAAGTTCTCTGACC |
| R: GGAATCAAAACGATCAAACTGCG | |
| CD8 | F: CTCTGGCTGGTCTTCAGTATGA |
| R: TCTTTGCCGTATGGTTGGTTT | |
| IL-6 | F: GGCCTTCCCTACTTCACAAG |
| R: ATTTCCACGATTTCCCAGAG | |
| IFNγ | F: ATGAACGCTACACACTGCATC |
| R: CCATCCTTTTGCCAGTTCCTC | |
| MCP-1 | F: CTTCTGGGCCTGCTGTTCA |
| R: CCAGCCTACTCATTGGGATCA | |
| TLR2 | F: TTGCTGGGCTGACTTCTCTCA |
| R: GAAGAGTCAGGTGATGGATGTCG | |
| TLR4 | F: TCAGAACTTCAGTGGCTGGATT |
| R: AACTCTGGATAGGGTTTCCTGTCA | |
| NPC-1 | F: GGGGCATCAGTTACAATGCT |
| R: AAACACCGCACTTCCCATAG | |
| Ang-1 | F: CTCGTCAGACATTCATCATCCA |
| R: CACCTTCTTTAGTGCAAAGGCT | |
| Ang-2 | F: CAGCCACGGTCAACAACTC |
| R: CTTCTTTACGGATAGCAACCGAG | |
| VEGF-A | F: GCACATAGAGAGAATGAGCTTC |
| R: CTCCGCTCTGAACAAGGCT | |
| VEGF-C | F: GTGAGGTGTGTATAGATGTGGG |
| R: GTCTTGCTGAGGTAACCTGTG | |
| VEGF-D | F: TCACGCTCAGCATCCCATC |
| R: ACTTCTACGCATGTCTCTCTAGG | |
| VEGFR-1 | F: TGGCTCTACGACCTTAGACTG |
| R: CAGGTTTGACTTGTCTGAGGTT | |
| VEGFR-2 | F: TTTGGCAAATACAACCCTTCAG |
| R: GCAGAAGATACTGTCACCACC | |
| VE-cadherin | F: CACTGCTTTGGGAGCCTTC |
| R: GGGGCAGCGATTCATTTTTCT | |
| β-Catenin | F: TCCCTGAGACGCTAGATGAGG |
| R: CGTTTAGCAGTTTTGTCAGCTC | |
| Gp91 phox | F: TATGCTGATCCTGCTGCCAGT |
| R: TGTCTTCGAATCCTTGTCGAGC | |
| Gapdh | F: CCAGGTTGTCTCCTGCGACT |
| R: ATACCAGGAAATGAGCTTGACAAAG |
See text for definitions.