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. 2013 Jun 21;305(4):H484–H493. doi: 10.1152/ajpheart.00642.2012

Fig. 2.

Fig. 2.

CaMKK inhibition reduces LS-induced increase in the expression and activity of KCa3.1 and KCa2.3. HCAECs were exposed to LS for 24 h in the absence or presence of the CaMKK inhibitor STO-609 (10 μg/ml). LS-induced increase in KCa3.1 (A) and KCa2.3 (B) mRNA expression was significantly suppressed by CaMKK inhibition. C: Western blotting revealed that increased protein expression levels of KCa2.3 and KCa3.1 under LS condition were inhibited by CaMKK inhibition. Top: a representative image of immunoblots. Bottom: the summaries of densitometric analysis of KCa3.1 and KCa2.3 (n = 3). D: representative KCa currents in HCAECs after LS exposure (top). KCa3.1 and KCa2 currents were sensitive to 1 μM TRAM 34 and 300 nM apamin, respectively. Summary of KCa3.1 and KCa2 currents densities at 40 mV in HCAECs before and after LS exposure with or without STO-609 (n = 5; bottom). LS-induced increase in the channel activities of KCa3.1 and KCa2 under LS condition was diminished by CaMKK inhibition. *Signficant difference vs. ST (P < 0.05). #Signficant difference vs. LS (P < 0.05).