Fig. 6.

Mitochondrial O₂ consumption and respiratory protein content. Rates of mitochondrial O₂ consumption in the presence of 5 mM pyruvate/2 mM malate (P/M, Complex I_min); P/M + 5 mM ADP (P/M_ADP, Complex I_max); P/M_ADP + 5 mM glutamate (P/M/G_ADP, Complex I_max); P/M/G_ADP + 5 mM succinate (P/M/G/S_ADP, Complex I + II_max); and P/M/G/S_ADP + 1 μM rotenone (Complex II_max) are shown in A. Shown in B are representative immunoblots (n = 4) of mitochondrial respiratory enzyme subunits of complexes I-IV + complex V (H⁺-ATPase) in naïve mPer2^−/− and WT myocardium; Values are means ± SE (n = 5). *P < 0.01 vs. all other groups for that condition.