Fig. 7.

Mitochondrial outer membrane integrity and ROS production. Intactness of the outer mitochondrial membrane was used as an index of the structural integrity of mitochondria by measuring the rates of maximal ADP-stimulated O₂ consumption before (−) and after (+) exogenous cytochrome c supported by pyruvate/glutamate/malate, as shown in A. Representative experimental traces of mitochondrial ROS production (H₂O₂) supported by pyruvate/glutamate/malate + 75 μM ADP are shown in B from all 4 experimental groups. Quantified rates of mitochondrial H₂O₂ production are presented in C. Values are means ± SE (n = 5). *P < 0.01 vs. all other groups for that condition. †P < 0.05, main effect of cytochrome c within group.