Table 2. Summary of the results of MGMT promoter methylation testing in cases with methylated MGMT promoter according to the reference laboratory. There is a perfect correlation (100% concordance) of MGMT status in all samples irrespective of tissue fixation conditions, testing laboratory, testing technician and time point of testing. Cut-offs and abbreviations: same as Table 1.
| ID | Test lab | Test run | Fixation | Method | Technician | Mean value | Result |
|---|---|---|---|---|---|---|---|
| Case 2 | Reference lab | 1 | RCLPE | MSRE | 1 | 0.56 | methylated |
| Case 2 | Sequenom | 2 | RCLPE | Epityper | 1 | 16.9 | methylated |
| Case 2 | Test lab 1 | 3 | FFPE | PSQ | 1 | 21.25 | methylated |
| Case 2 | Test lab 1 | 4 | FFPE | PSQ | 1 | 21.75 | methylated |
| Case 2 | Test lab 1 | 5 | FFPE | PSQ | 2 | 20.50 | methylated |
| Case 2 | Test lab 1 | 6 | RCLPE | PSQ | 1 | 26.25 | methylated |
| Case 2 | Test lab 1 | 7 | RCLPE | PSQ | 2 | 20 | methylated |
| Case 2 | Test lab 2 | 8 | FFPE | PSQ | 1 | 31.35 | methylated |
| Case 2 | Test lab 2 | 9 | RCLPE | PSQ | 1 | 25.64 | methylated |
| Case 3 | Reference lab | 1 | RCLPE | MSRE | 1 | 0.69 | methylated |
| Case 3 | Sequenom | 2 | RCLPE | Epityper | 1 | 20.9 | methylated |
| Case 3 | Test lab 1 | 3 | FFPE | PSQ | 1 | 28.5 | methylated |
| Case 3 | Test lab 1 | 4 | FFPE | PSQ | 1 | 30.25 | methylated |
| Case 3 | Test lab 1 | 5 | FFPE | PSQ | 2 | 34.75 | methylated |
| Case 3 | Test lab 1 | 6 | RCLPE | PSQ | 1 | 58 | methylated |
| Case 3 | Test lab 1 | 7 | RCLPE | PSQ | 2 | 50.75 | methylated |
| Case 3 | Test lab 2 | 8 | FFPE | PSQ | 1 | 36.21 | methylated |
| Case 3 | Test lab 2 | 9 | RCLPE | PSQ | 1 | 48.94 | methylated |
| Case 4 | Reference lab | 1 | RCLPE | MSRE | 1 | 7.35 | methylated |
| Case 4 | Sequenom | 2 | RCLPE | Epityper | 1 | 15 | methylated |
| Case 4 | Test lab 1 | 3 | FFPE | PSQ | 1 | 17.75 | methylated |
| Case 4 | Test lab 1 | 4 | FFPE | PSQ | 1 | 18.25 | methylated |
| Case 4 | Test lab 1 | 5 | FFPE | PSQ | 2 | 18.75 | methylated |
| Case 4 | Test lab 1 | 6 | FFPE | PSQ | 2 | 16.5 | methylated |
| Case 4 | Test lab 1 | 7 | FFPE | PSQ | 2 | 18.5 | methylated |
| Case 4 | Test lab 1 | 8 | RCLPE | PSQ | 1 | 55.25 | methylated |
| Case 4 | Test lab 1 | 9 | RCLPE | PSQ | 2 | 35.5 | methylated |
| Case 4 | Test lab 2 | 10 | FFPE | PSQ | 1 | 18.03 | methylated |
| Case 4 | Test lab 2 | 11 | RCLPE | PSQ | 1 | 34.94 | methylated |
| Case 6 | Reference lab | 1 | RCLPE | MSRE | 1 | 11.85 | methylated |
| Case 6 | Sequenom | 2 | RCLPE | Epityper | 1 | 23.4 | methylated |
| Case 6 | Test lab 1 | 3 | FFPE | PSQ | 1 | 20.25 | methylated |
| Case 6 | Test lab 1 | 4 | FFPE | PSQ | 1 | 20.5 | methylated |
| Case 6 | Test lab 1 | 5 | FFPE | PSQ | 2 | 15.25 | methylated |
| Case 6 | Test lab 1 | 6 | FFPE | PSQ | 2 | 14.5 | methylated |
| Case 6 | Test lab 1 | 7 | RCLPE | PSQ | 1 | 49 | methylated |
| Case 6 | Test lab 1 | 8 | RCLPE | PSQ | 2 | 38.25 | methylated |
| Case 6 | Test lab 2 | 9 | FFPE | PSQ | 1 | 24.42 | methylated |
| Case 6 | Test lab 2 | 10 | RCLPE | PSQ | 1 | 36.23 | methylated |
| Case 8 | Reference lab | 1 | RCLPE | MSRE | 1 | 69.38 | methylated |
| Case 8 | Sequenom | 2 | RCLPE | Epityper | 1 | 31.3 | methylated |
| Case 8 | Test lab 1 | 3 | FFPE | PSQ | 1 | 30.25 | methylated |
| Case 8 | Test lab 1 | 4 | FFPE | PSQ | 1 | 31.25 | methylated |
| Case 8 | Test lab 1 | 5 | FFPE | PSQ | 2 | 40.25 | methylated |
| Case 8 | Test lab 1 | 6 | RCLPE | PSQ | 1 | 48.75 | methylated |
| Case 8 | Test lab 1 | 7 | RCLPE | PSQ | 2 | 41.75 | methylated |
| Case 8 | Test lab 2 | 8 | RCLPE | PSQ | 1 | 37.75 | methylated |
| Case 8 | Test lab 2 | 9 | FFPE | PSQ | 1 | 33.98 | methylated |