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. 2014 Jan 14;34(1):e00089. doi: 10.1042/BSR20130091

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© 2014 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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Subconfluent HDF were either mock-treated (CM^HDF), treated with rTGFβ1 (10 ng/ml) for 48 h (CM^HDF,TGF) and in CM of squamous carcinoma cells SCL-1 (CM^SCL−1). (A) The amount of αSMA protein was immunostained for αSMA and (B) determined by Western blot analysis. The densitometric values represent the fold increase over control, which was set at 1.0. The data represent means±S.E.M. of three independent experiments. CM, conditioned medium.