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. 2014 Jan 14;34(1):e00089. doi: 10.1042/BSR20130091

Figure 2. TGFβ1-mediated expression of αSMA.

Figure 2

(A) Subconfluent HDFs were either mock-treated or pretreated for 24 h with allopurinol (10 μM) before addition of rTGFβ1 (10 ng/ml). TGFβ1 and the allopurinol were present for an additional 48 h. The level of αSMA protein was determined by Western blot. α-tubulin was used as loading control. Three independent experiments were performed. (B) HDF monolayer cultures were cultured in CMHDF containing apocynin (1 mM) for 1 h or DPI (5 μM) for 24 h before treatment with TGFβ1 (10 ng/ml) for further 48 h. The level of αSMA protein was determined by Western blot. α-tubulin was used as loading control. The experiments were performed in triplicate. (C) Subconfluent HDF were preincubated for 1 h with apocynin (1 mM) in serum-free medium and then TGFβ1 (10 ng/ml) treated for 24 h. Steady-state mRNA levels of αSMA were analysed by real-time RT-PCR. Data are given as means of three independent experiments±S.E.M.