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. 2014 Jan 14;34(1):e00089. doi: 10.1042/BSR20130091

Figure 4. rTGFβ1 activates the NADH oxidase in dermal fibroblasts.

Figure 4

(A) Rates of NADH consumption by ct and time course of the rates from HDF following rTGFβ1 (10 ng/ml) treatment. TPA was used as a positive control. In presence of 250 μM NADH, subconfluent HDF were either mock-treated or treated with rTGFβ1. The consumption of NADH was measured spectrophotometrically. data represent the mean±S.E.M. (B) Subconfluent HDF were preincubated for 1 h with apocynin (1 mM) in the serum-free medium and then rTGFβ1 (10 ng/ml) treated for various time points. p67phox and NOX4 mRNA expression were analysed by RT–PCR. HPRT1 was used as housekeeping gene. Three independent experiments were performed. (C) Subconfluent HDFs were either mock-treated, treated with rTGFβ1 (10 ng/ml) for 48 h or incubated with apocynin for 1 h or starting 4, 8 and 16 h after rTGFβ1 treatment. The level of αSMA protein was determined by Western blot. Coomassie Brilliant blue staining was used as loading control. Three independent experiments were performed.