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. 2014 Jan 13;6(1):e00133. doi: 10.1042/AN20130042

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© 2014 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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To confirm that the 3-NP treatment was effective, we examined the striatum and SCN of a cohort of treated mice. Using Nissl and hematoxylin and eosin staining, we found that the striatum exhibited pathological damage in all of the treated mice (n=10/10). To provide one measure, the ventricle volumes adjacent to the striatum as well as the SCN were measured. Top left: photomicrographs (4×) of striatum Nissl staining sections. Top right: averaged ventricle size of striatum. *P<0.05, Student's t test (vs control). Bottom left: photomicrographs (10×) of SCN Nissl staining sections. 3V, 3rd ventricle, OC, optic chiasm. Bottom right: averaged SCN size. Data are shown at means+S.E.M.