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. 2013 Dec 23;5(5):e00130. doi: 10.1042/AN20130023

Figure 6. dbcAMP-PKA-Notch signaling involvement in astrocytic differentiation of C6 cells.

Figure 6

C6 cells were treated with dbcAMP (750 μM) and, when indicated, DAPT (40 μM) or H89 (10 μM) or DAPT/H89 (20 μM/5 μM) inhibitors were added 30 min before dbcAMP stimuli. (A) [3H]D-aspartate uptake assays, where cells were harvested 24 h after treatment. (B) qRT-PCR to amplify GFAP mRNA cells (harvested 24 h after treatment). (C) Map of pGLF1-Luciferase reporter for testing GFAP promoter-dependent transcription. Cells were transfected with 0.5 μg of pGF1L reporter and 24 h after treated with dbcAMP (750 μM, 24 h). When indicated, before dbcAMP stimuli (30 min), cells were treated as in (A). Data are shown as relative to values recorded in non-treated (NT) control and plotted as means±S.E.M. from at least three independent experiments. Statistical analysis was performed using a non-parametric one-way ANOVA (Kurskal–Wallis test) and Dunn's post-hoc test (*P<0.05; **P<0.01; ***P<0.001).