Table 1.

Frequency of Oocyte Labeling Patterns Following In Vitro Fertilization

Probe	n	Under, attached, bound or fused sperm (%)	Over, incorporated sperm (%)	Over MII spindle (%)
anti-P-Tyr	64	31	82	65
anti-FAK PY861	54	0	17	15
anti-PYK2 PY579	70	14	75	87

Oocytes subjected to in vitro fertilization with and without zona removal were fixed at time points where sperm were in contact with, bound, or fused with the oocyte plasma membrane. Samples were labeled with anti-P-Tyr, anti-FAK PY⁸⁶¹, or anti-PYK2 PY⁵⁷⁹ as described in Materials and Methods. Confocal immunofluorescence microscopy was used to image the antibody labeling patterns. Data were collected from 16 experiments. (n) represents the number of oocytes examined within each antibody group.