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. Author manuscript; available in PMC: 2014 Sep 4.
Published in final edited form as: Reproduction. 2013 Oct 4;146(5):501–516. doi: 10.1530/REP-13-0311

FIGURE 5. Chromatin immunoprecipitation (ChIP) analysis of histone acetylation in Pgk2 transgenes.

FIGURE 5

(A). A map of the Pgk2-CAT transgenes shows the position of amplicons analyzed for acetyl-H3 or acetyl-H4 in Pgk2-CAT transgenes. (B) Relative enrichment of acetylated H4 (graphs on the left) or acetylated H3 (graphs on the right) is shown for each transgene as well as for the two control genes, ß-actin and Cryaa in spleen cells or mixed male germ (spermatogenic) cells. The 515 Pgk2-CAT/WT transgene, which is actively expressed in spermatogenic cells, shows significant enrichment for both acetyl-H3 and acetyl-H4 in male germ cells, but not in spleen cells where this transgene is not expressed. The 515 Pgk2-CAT/CAAT-minus transgene, which contains all regulatory sequences normally required to direct testis-specific expression except for an intact CAAT-box in the core promoter, and is not expressed in either spleen cells or spermatogenic cells, shows no enrichment of acetyl-H3 or acetyl-H4 in either cell type. Similarly, the 188 Pgk2-CAT transgene, which contains only the Pgk2 core promoter and no enhancer region and is not expressed in either spleen or spermatogenic cells, shows no enrichment for either histone modification in either cell type.