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. Author manuscript; available in PMC: 2014 Jan 14.
Published in final edited form as: Clin Immunol. 2010 May 15;136(3):329–337. doi: 10.1016/j.clim.2010.04.014

Figure 3.

Figure 3

CD4+CD25+ Treg cells isolated from the peripheral blood of patients with immune-mediated diseases can be expanded 100–2000 fold ex vivo with sustained purity. Human CD4+CD25+ Treg cells enriched from the peripheral blood of patients with immune-mediated diseases were activated and expanded ex vivo with Dynal CD3/28 T cell expander beads and recombinant IL-2 as described. Cell numbers on day 7, 14 and 21 cultures were counted for each individual culture. The ex vivo Treg cell growth curves were shown in A. Each line represents a single subject. The purity of ex vivo expanded human Treg cells at week 2 was determined by intracellular Foxp3 staining, and was shown in B. Each dot represents one subject and the bar represents the average purity of expanded Treg cells (Fig. 3B, *p<0.05, Mann Whitney test for two group comparison).