Table 1. ApoE isoform and neuron age effects on spine subclasses.
Values shown are density per 20 μm dendrite lengths, n equals average of 50 dendrite segments/isoform per time point. Data shown are representative of two independent experiments. # denotes significant differences at P<0.05, between E2 and E3, § between E2 and E4, and * between E3 and E4, one-way ANOVA and Tukey's post-hoc test. Values are means±S.E.M. Phases: formation, DIV10–18; maintenance, DIV18–21; and loss, DIV21–26
| Type | DIV10 | DIV14 | DIV18 | DIV21 | DIV26 |
|---|---|---|---|---|---|
| Mature spines | |||||
| GluN1+GluA2 | # § | * § | # | ||
| ApoE2 | 5.0±0.4 | 9.2±1.1 | 12.8±0.7 | 13.4±0.5 | 8.8±0.7 |
| ApoE3 | 4.9±0.4 | 6.5±0.5 | 12.5±0.5 | 11.6±0.6 | 6.4±0.4 |
| ApoE4 | 3.9±0.3 | 5.8±0.4 | 12.7±0.5 | 9.6±0.4 | 7.8±0.3 |
| Immature spine types | |||||
| GluN1-only | # | * | # * | * | * |
| ApoE2 | 6.0±0.7 | 7.6±1.0 | 15.5±1.3 | 15.4±0.7 | 13.7±0.8 |
| ApoE3 | 4.1±0.4 | 8.5±0.6 | 12.1±0.6 | 14.0±0.6 | 15.9±0.8 |
| ApoE4 | 4.8±0.4 | 5.4±0.4 | 14.7±0.7 | 16.5±0.6 | 12.1±0.6 |
| GluA2-only | * | # * | # § | § | |
| ApoE2 | 3.2±0.3 | 5.6±0.7 | 7.0±0.5 | 6.3±0.4 | 3.3±0.4 |
| ApoE3 | 2.7±0.3 | 7.4±0.6 | 5.1±0.3 | 4.6±0.3 | 2.8±0.3 |
| ApoE4 | 2.8±0.2 | 3.8±0.3 | 7.2±0.4 | 4.7±0.2 | 2.2±0.2 |
| Drebrin-only | § | # | |||
| ApoE2 | 5.7±0.6 | 3.1±0.8 | 4.7±0.9 | 5.5±0.7 | 8.5±0.8 |
| ApoE3 | 4.4±0.4 | 5.5±0.7 | 5.5±0.7 | 7.6±0.6 | 10.8±0.6 |
| ApoE4 | 4.4±0.4 | 4.7±0.5 | 4.0±0.6 | 8.9±0.5 | 9.8±0.5 |