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. 2014 Jan 14;12(1):e1001762. doi: 10.1371/journal.pbio.1001762

Figure 2. In vivo homeostasis of A20-deficient mast cells.

Figure 2

(A) Representative immunofluorescence images of whole-mount ear explants: green, avidin-FITC; red, anti-laminin; scale bar, 100 µm. Scatter plot shows proportions of mast cells (CD45+FcεRI+c-Kit+) in ear skin digests as identified by flow cytometric analysis. Bars indicate means from at least eight mice per genotype (Control, 7 Mcpt5Cre, 3 Cre− littermates). (B) Dot plots showing proportions of ex vivo isolated peritoneal mast cells (FcεRI+c-Kithi). Numbers represent means ± SD from at least 20 mice per genotype (Control, 15 Mcpt5Cre, 5 Cre− littermates). (C) Median fluorescent intensities (MFIs) of various cell surface markers expressed on A20-deficient and control ex vivo isolated peritoneal mast cells. Data are means + SD from at least four mice per genotype (Control, 4 Mcpt5Cre, 1 Cre− littermate). (D) Scatter plots show absolute numbers of peritoneal mast cells (FcεRI+c-Kithi) and total peritoneal cells. Bars indicate means from at least 10 mice per genotype (Control, 8 Mcpt5Cre, 5 Cre− littermates). (E) Scatter plots show proportions of cytokine positive peritoneal mast cells (c-Kit+) as identified by intracellular flow cytometric analysis. Bars indicate means from at least eight mice per genotype (Control, 9 Mcpt5Cre, 2 Cre− littermates). *p<0.05, **p<0.01, ***p<0.001 (one-way ANOVA).