Table 1. Antibody cell surface affinity.

	Anti-Her2 Cys-ADC	Anti-Her2 position 1-pAF	Anti-Her2 position 1-ADC	Anti-Her2 position 2-ADC	Anti-Her2 (wild type)	Anti-CD22 position 1-ADC	Anti-CD22 (wild type)
Bmax	1272	1353	1262	1668	1497	3.5	3.7
Kd (nM)	1.5	2.1	1.9	1.7	2.4	No binding	No binding

Antibody affinity was measured using HCC-1954 cells. The maximum binding and antibody affinity measurements were obtained for each antibody. HCC-1954 cells do not express CD22 therefore the anti-CD22 antibodies did not bind to the HCC-1954 cells.