Figure 6. Generation of hybrid proteins consisting of a backbone derived from the TM protein p15E of PERV and the FPPR and MPER of gp41 of HIV-1, which replace the FPPR and MPER of p15E. The FPPR and MPER of p15E were removed and the FPPR and MPER (containing the epitopes of the broadly neutralizing antibodies 2F5 and 4E10) of gp41 of HIV-1 were inserted. NHR - N-terminal helical region, C - C or C - CC - Cys-Cys-loop, CHR - C-terminal helical region, MSD - membrane spanning domain.