Table 1. ELISPOT assay for IFN-γ antigen-specific responses in the spleens of vaccinated mice following stimulation with HSP65 protein.
| Vaccination | Number of IFN-γ secreting cells/ (106splenocytes) |
|||
|---|---|---|---|---|
| (1st/2nd/3rd) | ||||
| G1 | −/−/- | 3 | ± | 1 |
| G2 | −/−/BCG | 13 | ± | 6 |
| G3 | DNA* / DNA / DNA | 16 | ± | 4 |
| G4 | BCG / DNA / DNA | 115 | ± | 12 |
*DNA means HVJ-Envelope/HSP65 DNA + IL-12 DNA vaccine. Spleen cell cultures were stimulated with rHSP65 protein for 20 h. The number of IFN-γ-secreting cells specific for rHSP65 protein per million cells was determined individually by ELISPOT assay. Results are expressed as the mean ± S.D. of 6 wells of 3 mice per group. The statistically significant differences of the G1 (naïve) group compared with the G2 (BCG alone group), G3 (DNA / DNA / DNA) or G4 (BCG / DNA / DNA) were observed (p < 0.01). The statistically significant difference between G2 group and G4 was observed (p < 0.01). The statistically significant difference between G3 group and G4 was observed (p < 0.01).