Figure 7. Proliferation of PBL from monkeys vaccinated with HVJ-Envelope/15K granulysin DNA by the stimulation with HSP65 antigen. Five × 102 M. tuberculosis (Erdman strain) were intratracheally into cynomolgus monkeys as described in Materials and Methods. Four weeks after challenge of TB, 400μg of HVJ-Envelope/15K granulysin were injected i.m. Six times every two weeks. The proliferation of PBL from monkeys vaccinated with HVJ-Envelope/15K granulysin on 13 weeks after TB challenge were assessed by the 3H-TdR uptake of lymphocyte for 3 d culture.