Table 1. Antibody reactivity of rat anti-OMP26 peptide against OMP26 protein and peptides.
| Immunogen | Antibody reactivity against: | |||
|---|---|---|---|---|
| Homologous peptide assayed by | OMP26 protein assayed by | |||
| ELISAa | SPRb | ELISAa | SPRb | |
| T1 | 440.3 | 502.2 | 13.6 | 7.9 |
| T2 | 25.5 | 730.8 | 297.4 | 56.5 |
| T3 | 209.1 | 1410 | 2230.7 | 301.2 |
| T4 | 2350.8 | 1896.6 | 7682.1 | 1052 |
| T1a+T2 | 3915.7 | 1783.5 | 5746.6 | 599.1 |
| T2+T3 | 1119.5 | 1812.6 | 2424.9 | 400.2 |
| T3+T4 | 5290.9 | 807.4 | 6109.6 | 940.4 |
a Antibody reactivities are expressed as IgG concentration in μg/ml. The assay was performed in duplicate, and the values represent the mean reactivity of immune sera from 3–5 rats. Non-immune sera did not react to any of the OMP26 peptides, and only slight reactivity to the OMP26 protein was detected at the dilution 1:25 (results not shown).bSPR as expressed by Resonance Units.