Table 2. Oligonucleotide primers used in PCR to construct OMP26 peptides.

Primer	Nucleotide positiona	Sequenceb
T1F	1–21	5′GGC GGA TCC ATG AAA AAC ATC GCA AAA GTA 3′
T1aF	70–90	5′ GGC GGA TCC GAA GAA AAA ATT GCT TTC ATT 3′
T1HindR	145–165	5′ GGC AAG CTT AGG TTT AAA TTC AGC ATC AAG 3′
T2F	133–153	5′ GGC GGA TCC GTA GCA GAT AAA CTT GAT GCT 3′
T2HindR	280–300	5′ GGC AAG CTT GCG TTT TTG AAT ATC AGC TTG 3′
T3F	283–303	5′ GGC GGA TCC GCT GAT ATT CAA AAA CGC CAA 3′
T3HindR	415–435	5′ GGC AAG CTT TAA TAA TTT ACC ACG TTC TTC 3′
T4F	418–438	5′ GGC GGA TCC GAA CGT GGT AAA TTA TTA GAT 3′
T4HindR	571–591	5′ GGC AAG CTT TTT TTT CTC TTG TGC TTT TTC 3′

^a Nucleotide position in the omp26 gene from NTHi-289; ^bRecognition sites for restriction endonucleases BamHI and HindIII are underlined