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. 2014 Jan 14;9(1):e85206. doi: 10.1371/journal.pone.0085206

Figure 2. Expression of TMCC1 protein in human cells.

Figure 2

(A) Whole cell extracts of HeLa cells were collected and immunoblotted with pre-immune serum or TMCC1 antibody. (B) HeLa cells were transfected with TMCC1 siRNA (siTMCC1-1 or siTMCC1-2) or a control siRNA; 72 h post-transfection, whole cell extracts were prepared and immunoblotted for TMCC1. α-Tubulin was stained as a control. (C) Whole cell extracts of various human cell lines were collected and immunoblotted for TMCC1; β-actin was stained as a control.