Table 1. Distinguishing properties of low, high, and very high [PIN+] variants.
Distinguishing characteristics of [PIN+] variants | Low [PIN+] | High [PIN+] | Very high [PIN+] | |
---|---|---|---|---|
Effects on [PSI+] | Efficiency of [PSI+] induction | + | +++ | ++++ |
Predominant [PSI+] variant induced in vivo at 30 °C | weak | weak | strong | |
Predominant [PSI+] variant induced in vivo at 4 °C | Not tested | strong | Not tested | |
Other features | Level of soluble Rnq1 | ++ | + | +++ |
Single (s.d.) or multi (m.d.) fluorescent Rnq1:Gfp dots | s.d. | m.d. | s.d. | |
Average [PIN+] seed (propagon) number/cell | 25 ± 5 | 96 ± 17 | 50 ± 5 | |
Oligomers break into sub-particles at 60 °C12 | no | yes | no |